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Secondary Antibodies

Secondary antibodies bind to a primary antibody to assist in detection, amplification, sorting, and purification of target antigens, and are used in various biochemical techniques/assays (e.g., ELISA, western blot (WB), immunohistochemistry).
Goat Secondary Antibodies (44,221)
Mouse Secondary Antibodies (14,558)
Rabbit Secondary Antibodies (6,314)
Donkey Secondary Antibodies (4,319)
Rat Secondary Antibodies (1,238)
Sheep Secondary Antibodies (701)
Chicken Secondary Antibodies (403)
Bovine Secondary Antibodies (258)
Pig Secondary Antibodies (78)
Horse Secondary Antibodies (74)
Alpaca Secondary Antibodies (62)
Hamster Secondary Antibodies (57)
Biosimilar Secondary Antibodies (9)
Human Secondary Antibodies (7)
Duck Secondary Antibodies (3)

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7,021 – 7,035 of 72,106 results
7,021

Donkey anti-Goat IgG (H+L) Secondary Antibody, FITC, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4C. Do not freeze.
Target Species Goat
Host Species Donkey
Conjugate FITC
Applications Flow Cytometry,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Frozen),Immunohistochemistry (Paraffin)
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration 1.0 mg/mL
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:200 - 1:2, Immunohistochemistry-Paraffin 1:200 - 1:2
Classification Polyclonal
Immunogen Goat IgG-heavy and light chain
Primary or Secondary Secondary
Test Specificity By immunoelectrophoresis and ELISA this reacts specifically with goat IgG and with light chains common to other goat immunoglobulins. No was detected against non-immunoglobulin serum proteins. This may cross react with IgG from other species.
7,022

Donkey anti-Sheep IgG (H+L) Secondary Antibody, DyLight 800 (Pre-adsorbed), Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Target Species Sheep
Host Species Donkey
Conjugate DyLight 800
Applications Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:10000, Immunocytochemistry/Immunofluorescence 1:5000, Fluorophore-linked immunosorbent assay 1:20000
Classification Polyclonal
Immunogen Sheep IgG whole molecule
Reconstitution Reconstitute with 100 ul deionized water (or equivalent).
Primary or Secondary Secondary
Test Specificity This antibody was pre-adsorbed against Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, and Rat Serum Proteins. No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit or Rat Serum Proteins. This antibody will react with heavy chains of Sheep IgG and with light chains of most Sheep immunoglobulins.
7,023

Donkey anti-Rabbit IgG (H+L) Secondary Antibody, HRP (Pre-adsorbed), Novus Biologicals™
SDP

Donkey Polyclonal Antibody has been used in 2 publications

Content And Storage Store at 4C. Do not freeze.
Target Species Rabbit
Host Species Donkey
Conjugate HRP
Applications ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Simple Western,Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration 0.5 mg/mL
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:5000 - 1:50000, Simple Western reported by internal validation, ELISA 1:10000-1:100000, Immunohistochemistry 1:200 - 1:5000, Immunocytochemistry/Immunofluorescence 1:200 - 1:5000, Immunohistochemistry-Paraffin 1:200 - 1:5000
Classification Polyclonal
Immunogen Rabbit IgG-heavy and light chain
Primary or Secondary Secondary
Test Specificity By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No antibody was detected against nonimmunoglobulin serum proteins. Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive Antibodies. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat IgG was detected. This antibody may cross react with IgG from other species.
7,024

Donkey F(ab) anti-Goat IgG (H+L) Secondary Antibody, Texas Red, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Target Species Goat
Host Species Donkey
Conjugate Texas Red
Applications Flow Cytometry,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Flow Cytometry 1:1:10 - 1:1000, Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000, Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
Classification Polyclonal
Immunogen Goat IgG whole molecule
Reconstitution Reconstitute with 1.0 ml deionized water (or equivalent).
Primary or Secondary Secondary
Test Specificity No reaction was observed against anti-Papain or anti-Donkey IgG F(c).
7,025

Donkey anti-Sheep IgG (H+L) Secondary Antibody, PE, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Sheep
Host Species Donkey
Conjugate PE
Applications ELISA,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Multi-step
Formulation PBS
Classification Polyclonal
Immunogen Donkey anti-Sheep IgG (H+L) Secondary Antibody (Pre-adsorbed) was produced by repeated immunization with Sheep IgG whole molecule in donkey.
Primary or Secondary Secondary
7,026

Donkey anti-Rabbit IgG (H+L) Secondary Antibody, DyLight 650, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Rabbit
Host Species Donkey
Conjugate DyLight 650
Applications ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Immunogen affinity purified
Formulation 50mM Sodium Borate
Classification Polyclonal
Immunogen This Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) was developed against rabbit IgG.
Primary or Secondary Secondary
7,027

Invitrogen™ Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Biotin

Donkey Polyclonal Secondary Antibody

ENCOMPASS_PREFERRED
Content And Storage Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target Species Rabbit
Host Species Donkey
Conjugate Biotin
Applications ELISA,Flow Cytometry,Western Blot,Immunocytochemistry
Form Liquid
Isotype IgG
Concentration 1.5 mg/mL
Antigen Rabbit IgG (H+L) Highly Cross-Adsorbed
Regulatory Status RUO
Purification Method Affinity chromatography
Product Type Secondary Antibody
Formulation PBS with 1% BSA and 0.05% sodium azide; pH 7.2
Immunogen Gamma Immunoglobins Heavy and Light chains.
Classification Polyclonal
Primary or Secondary Secondary
7,029

Donkey anti-Rabbit IgG (H+L) Secondary Antibody [Janelia Fluor 525], Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Rabbit
Host Species Donkey
Conjugate Janelia Fluor 525
Applications Western Blot,ELISA,Immunohistochemistry,Immunocytochemistry/Immunofluorescence,Immunohistochemistry (Paraffin)
Form Purified
Isotype IgG
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Immunogen affinity purified
Formulation 50mM Sodium Borate
Classification Polyclonal
Immunogen This Donkey anti-Rabbit IgG (H+L) Secondary Antibody was developed against rabbit IgG-heavy and light chain.
Primary or Secondary Secondary
7,030

Donkey anti-Mouse IgG (H+L) Secondary Antibody, Allophycocyanin, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Mouse
Host Species Donkey
Conjugate APC
Applications Chemiluminescence Immunoassay (CIA),ELISA,Flow Cytometry,Immunoassay,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunomicroscopy,Western Blot
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Formulation PBS
Classification Polyclonal
Immunogen This Donkey anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) was developed against purified mouse IgG (H&L).
Primary or Secondary Secondary
7,031
Promotions Available

Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody, R&D Systems™
GREENER_CHOICE SDP

Donkey Polyclonal Antibody has been used in 5 publications

7,032

Donkey anti-Mouse IgG (H+L) Secondary Antibody, DyLight 594 (Pre-adsorbed), Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store lyophilized antibody at 4°C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20°C or below for long term storage.
Target Species Mouse
Host Species Donkey
Conjugate DyLight 594
Applications Flow Cytometry,Immunomicroscopy
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Dilution Flow Cytometry 1:20 - 1:2000, Immunomicroscopy 1:20 - 1:2000
Classification Polyclonal
Immunogen Purified Mouse IgG (H&L)
Reconstitution Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Primary or Secondary Secondary
Test Specificity Based on IEP, this antibody reacts with heavy gamma chains on mouse IgG and light chains on all mouse immunoglobulins. This antibody has been pre-adsorbed against bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat and sheep IgG.
7,033

Donkey anti-Rabbit IgG (H+L) Secondary Antibody, Rhodamine, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Target Species Rabbit
Host Species Donkey
Conjugate Rhodamine
Applications Flow Cytometry,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Western Blot
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Multi-step
Dilution Western Blot 1:1000 - 1:10000, Flow Cytometry 1:500 - 1:2500, Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000, Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
Classification Polyclonal
Immunogen Rabbit IgG whole molecule
Primary or Secondary Secondary
Test Specificity Prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti- Fluorescein, anti-Donkey Serum, Rabbit IgG and Rabbit Serum.
7,034

Donkey anti-Sheep IgG (H+L) Secondary Antibody, DyLight 633 (Pre-adsorbed), Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store lyophilized antibody at 4°C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20°C or below for long term storage.
Target Species Sheep
Host Species Donkey
Conjugate DyLight 633
Applications Flow Cytometry,Immunomicroscopy
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Dilution Flow Cytometry 1:20 - 1:2000, Immunomicroscopy 1:20 - 1:2000
Classification Polyclonal
Immunogen Purified Sheep IgG (H&L)
Reconstitution Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Primary or Secondary Secondary
Test Specificity Based on IEP, this antibody reacts with heavy gamma chains on sheep IgG and light chains on all sheep immunoglobulins. This antibody has been pre-adsorbed against human, mouse, and rabbit IgG.
7,035

Donkey anti-Rabbit IgG (H+L) Secondary Antibody, HRP (Pre-adsorbed), Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store lyophilized antibody at 4°C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20°C or below for long term storage.
Target Species Rabbit
Host Species Donkey
Conjugate HRP
Applications ELISA,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Dilution Western Blot 1:200-1:5000, ELISA 1:200-1:5000, Immunohistochemistry 1:500-1:5000, Immunohistochemistry-Paraffin
Classification Polyclonal
Immunogen Purified Rabbit IgG (H&L)
Reconstitution Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Primary or Secondary Secondary
Test Specificity Based on IEP, this antibody reacts with heavy gamma chains on rabbit IgG and light chains on all rabbit immunoglobulins. This antibody has been pre-adsorbed against mouse IgG and serum proteins.